TEL/AML1 dual-color double fusion probe aims to detect the translocation of the ETV6 (TEL) gene in chromosome 12p13.2 region and the RUNX1 (AML1) gene in the region of chromosome 21q22.12. The t(12;21)(p13.2;q22.1) translocation leads to the fusion of ETV6/RUNX1, the most common genetic recombination in patients with acute lymphoblastic leukemia (ALL) and is associated with a good prognosis. It is the highest incidence of childhood leukemia. In pediatric leukemia, acute lymphoblastic leukemia accounts for about 75%. In children with acute lymphoblastic leukemia aged 2-10, the positive rate of ETV6/RUNX1 (TEL/AML1) gene fusion accounts for about 20-25%, among which female is higher than male.
TEL probe uses an orange-red fluorescein label, and AML1 probe uses a green fluorescein label. The two probes combine to the target detection site by in situ hybridization. Under normal conditions (TEL/AML1 gene is not fused), it shows two orange-red signals and two green signals under a fluorescence microscope. When there is fusion, the green and orange-red signals form a yellow fusion signal due to recombination.
TEL/AML1 gene fusion has a 20-25% incidence in children with B-ALL. It has a good prognosis, but is prone to recurrence.
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Product size: 100μL FISH probe ( ⬤ | ⬤ ) + Pretreatment reagent (10 tests) + Antifade staining solution DAPI (10 tests).