BCL6 gene is located at the 3q27 region, and the protein encoded by the BCL6 gene is a transcriptional repressor involved in the development and function of the lymphatic system. Chromosome recombination of the BCL6 gene region is present in different types of non-Hodgkin’s lymphoma (NHL), including diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL). The most common translocation t(3;14)(q27;q 32.3) of BCL6 led to fusion of the IGH-BCL6 gene. Therefore, detection of BCL6 rearrangement by fluorescence in situ hybridization may be helpful in predicting clinical outcomes in patients with NHL (non-Hodgkin’s lymphoma).
BCL6 is a dual-color break apart probe composed of two probes directly labeled to 3q27.3-q28. The green-labeled fluorescent probe directly hybridizes with the 3q27.3 proximal BCL6 gene, while the orange-red labeled fluorescently probe directly hybridizes with the distal end of the BCL6 gene at the 3q27.3-q28 distal group.
In diffuse large B-cell lymphoma, BCL6 gene can translocate with multiple genes, the incidence rate is 20%-40%; in follicular lymphoma, the incidence rate is 5-15%. Burkitt’s lymphoma is morphologically suggestive of typical age, morphology, and immune characterization. If any of these three features is not typical or has a history of follicular lymphoma, and is accompanied by MYC gene breaks and BCL6 gene breaks, it should be diagnosed as a grey-area lymphoma between Burkitt and DLBCL. This probe aims to detect whether the BCL6 gene is broken and translocated. The BCL6 gene break is an independent indicator for evaluating survival rate and recovery rate.
Product size: 100μL FISH probe ( ⬤ | ⬤ ) + Pretreatment reagent (10 tests) + Antifade staining solution DAPI (10 tests).